You already mentioned TwoKinds, so I'll skip that one and get to some deeper cuts:
Night Physics: This is one of my all time favorites. Simply described by the author as: "a comic about a bear and his friends." A slice-of-life that contemplates the intricacies of navigating life in your 20's. (CW for: mature themes, drug use).
Pixeas and Henry: A story about two unlikely friends struggling for acceptance and coming to terms with themselves and their lives. (CW for: mature themes, implied SA, drug use, one of the characters struggles against homophobia, both internalized and external.)
Synthetic Instinct: From the author: "A Cyberpunk-Dystopia. Isa's mundane life is completely changed when the government finds out that she is a chimera- a hybrid of two unrelated genus. Navigating an unforgiving society Isa befriends a demobilized war machine named Rex, whose past is much darker and violent than she may realize. A story about who you are versus what the world wants you to be. Updates once a month."
Derideal: A series of sci-fi comics (there are several side stories set in the same universe available to read through) featuring anthro animals created as test projects for a shadowy corporate-state.
Project Roar: Not sure how to describe this one...A speculative historical fiction/sci-fi about a group of soldiers in WWII, biological experimentation, and their lives and experiences after the war. The story is still unfolding, but this is the general theme of the comic so far...
The Slow Decline: From the author: "When the world ended, they happened to be out of town. Now two co-workers, Rudy and Tom, are alone in a city that is making less sense by the day. The Slow Decline follows the two as they make their way through the mundane life of surviving the apocalypse. From anomalies at the breakfast table, smoke falling from the sky in the afternoon, or disembodied screaming through the night, it's all just another day at the end of it all. Updated EVERY THURSDAY!"
Psychopomp: From the author: "Something spooky’s happening in the eerie Southern town of Bonaventura, and Ray’s the only mutt who can communicate with the restless spirits plaguing the town. This ability might help him save the town from a supernatural threat, but it can’t help him navigate his own mundane life. Psychopomp is a queer comic which blends the campy mystery fun of Gravity Falls with the atmospheric horror of Silent Hill."
Lackadaisy: I'm sure you're already familiar with the animation, but before the animation, there was this comic. Beautifully illustrated and painstakingly historically researched, the comic follows a down-on-its-luck crew of rum-runners and the speakeasy they supply in prohibition-era St. Louis.
And for dessert, the saccharine sweet Duncan and Eddie: From the author: "Two boyfriends stumbling their through life together. They may not be the most competent couple, but that won't stop them from loving each other to bits! Updates every SUNDAY"